Reporter

Part:BBa_K5319674:Design

Designed by: YuFei Xie   Group: iGEM24_ZJU-China   (2024-09-30)


pJUMP42x-2a-TRS/RS-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 10
    Illegal NheI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 147


Design Notes

The 14-nt recruiting sequence is modified from the sequence in "Hydrolytic endonucleolytic ribozyme (HYER)is programmable for sequence-specific DNA cleavage" corresponding to the loxp site we want it to recognize.


Source

HYER(hydrolytic endonucleolytic ribozymes), identified from group II-C introns in public bacterial genome database in the article "Hydrolytic endonucleolytic ribozyme (HYER)is programmable for sequence-specific DNA cleavage".

References